2 edition of Design and characterisation of a bisensor for the detection of brain glutamate found in the catalog.
Design and characterisation of a bisensor for the detection of brain glutamate
Michael R. Ryan
|Statement||by Michael R. Ryan.|
|Contributions||University College Dublin. Department of Chemistry.|
|The Physical Object|
|Pagination||(5), vii, 197, (11), 24, (6)p. :|
|Number of Pages||197|
Design and Construction of a Distributed Sensor NET for Biotelemetric Monitoring of Brain Energetic Metabolism using Microsensors and Biosensors Pier Andrea Serra, Giulia Puggioni, Gianfranco Bazzu, Giammario Calia, Rossana Migheli and Gaia Rocchitta University of Sassari Italy 1. Introduction. Glutamate is an amino acid with very different functions: in the pancreas, it modulates the activity of the pancreatic ß--cells responsible for insulin production, whereas in the brain it is the.
The resulting biosensors show adequate characteristics in sensitivity, detection limit as well as functional and storage lifetimes for acute monitoring of the neuro-transmitters in brain tissue. The results of an extensive in vitro assessment and in vivo functional tests are : Olivier Frey. The central goal of this book is to broadly review the modem techniques and significant applications of chemical sensors and biosensors. Chapters are written by experts in the field - including Professor Joseph Wang, the most cited scientist in the world and renowned expert on .
A new class of optical biosensors based on the unique properties of photonic crystals has been recently developed by the Cunningham Group and by SRU Biosystems, a company co-founded by Prof. Cunningham in A photonic crystal label-free biosensor is comprised of a periodic arrangement of dielectric material in two or three dimensions. glutamate by boosting a natural process that the healthy brain already uses to safeguard itself from a glutamate overdose. If this concept is borne out in clinical trials, it might be helpful in treating a variety of acute and chronic brain insults and diseases.
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Monitoring the concentration level of brain glutamate via an implantable microbiosensor is a promising alternative approach to closely investigate in the function of glutamate as a neurotransmitter.
This review outlines glutamate microbiosensor designs to enhance the sensitivity of glutamate detection with less biofouling occurrence and minimal detection of interference by: 8. A smaller design would not only minimize the tissue damage during biosensor implantation and removal, but also would allow more precise targeting of brain regions.
In this study, we developed a microelectrode that is only 50 µm in diameter for measuring glutamate in vivo and in brain by: 8.
Amperometric l-glutamate (Glu) biosensors, based on both wild-type and a recombinant form of l-glutamate oxidase (GluOx), were designed and characterized in terms of enzyme-kinetic, sensitivity and stability parameters in attempts to fabricate a real-time Glu monitoring device suitable for future long-term detection of this amino acid in biological and other complex by: 6.
The design, construction, and optimization of enzyme-based electrochemical biosensors for in vivo and in vitro detection of glutamate are active areas of interdisciplinary research.
O’Brien KB, Killoran SJ, O’Neill RD, Lowry JP () Development of a catalase based biosensor for the detection of endogenous hydrogen peroxide in brain extracellular fluid. In: Di Chiara G, Carboni E, Valentini V, Acquas E, Bassareo V, Cadoni C (eds) Monitoring molecules in by: 2.
To improve signal transduction, various functional nanomaterials 37–40 and redox mediators 41–43 were incorporated into the electrode design. 44 Using these general strategies, Glu biosensors were utilized to investigate the dynamics of this messenger in the brain.
Glutamate in sleep/wake and reward. Over the past couple of years, enzyme based biosensors have been used to investigate processes related to L-glutamate release/uptake and the glucose cycle within the brain. However, most of these sensors, based on oxidoreductase enzymes, do not work in conditions of limited oxygen availability.
The electrode surface was a μm Pt/Ir wire that was mm in length. The design achieved a sensitivity of ± It was found that the response was oxygen independent up to μM ᴅ-serine. The limit of detection was determined to be ± μM and the biosensor has a response time of ± : Kenneth W.
Pierce. Mehta et al. create single-fluorophore kinase activity sensors for PKA, PKC and ERK in different colours, and demonstrate that they enable multiplex imaging of a combination of signalling Cited by: Microelectrode biosensors are a promising technique to probe the brain interstitial fluid and estimate the extracellular concentration of neurotransmitters like glutamate.
Their selectivity is largely based on maintaining high substrate specificity for the enzymes immobilized on by:  It can be used as a gene delivery vehicle , a contrast agent for imaging [38,39], and a biosensor for glutamate detection.
Mikeladze E, Collins A, Sukhacheva M, Netrusov A, Csoregi E () Characterization of a glutamate biosensor based on a novel gutamate oxidase integrated into a redox hydrogel. Electroanalysis – CrossRef Google ScholarCited by: 5. Neuronal communication relies on vesicular neurotransmitter release from signaling neurons and detection of these molecules by neighboring neurons.
Glutamate, the main excitatory neurotransmitter in the mammalian brain, is involved in nearly all brain functions. However, glutamate has suffered from detection schemes that lack temporal and spatial resolution allowed by by: 3.
Authoritative and practical, Biosensors and Biodetection: Methods and Protocols Volume 2: Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail.
The aim of the book is to make biosensors more. A method is described for construction of a highly sensitive electrochemical biosensor for detection of glutamate. The biosensor is based on covalent immobilization of glutamate oxidase (GluOx) onto polypyrrole nanoparticles and polyaniline composite film (PPyNPs/PANI) electrodeposited onto Cited by: Object.
In a companion study, the authors describe the development of a new instrument named the Wireless Instantaneous Neurotransmitter Concentration System (WINCS), which couples digital telemetry with fast-scan cyclic voltammetry (FSCV) to Cited by: At an applied potential of + mV (vs.
Ag/AgCl), a lower detection limit of approximately mM and an apparent Michaelis-Menten constant, K M app, of mM were determined. Key Words: biosensor, glutamate, oxidase, redox polymer, electron transfer relaysCited by: Electrochemical enzymatic biosensors are the subject of research due to their potential for in vivo monitoring of glutamate, which is a key neurotransmitter whose concentration is related to healthy brain function.
This study reports the use of biocompatible oxidised carbon nanohorns (o-CNH) with a high surface area, to enhance the immobilization of glutamate oxidase (GluOx) for improved Author: Rochelle Ford, Stephen. Devereux, Susan. Quinn, Robert.
O'Neill. Production of glutamate biosensor. BL21(DE3) bacteria were transformed with pRSET-FLII 81 Eμ plasmids (Okumoto et al., ; Deuschle et al., ) and streaked on an LB agar plate with ampicillin ( μg/ml).After overnight incubation at 37°C, a single colony was picked and grown in 1L LB with ampicillin ( μg/ml) for 2 days at 21°C in the dark with rapid shaking ( rpm).Cited by: Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter that is essential for normal brain function.
It is involved in multiple neuronal activities, including plasticity, information processing, and network synchronization. Abnormal GABA levels result in severe brain disorders and therefore GABA has been the target of a wide range of drug by: 7.
Enzymatic Biosensor for in vivo Applications Pedro Salazar, Miriam Martín, José Luis González–Mora A large number of analytical devices have recently been developed for detecting biomarkers without the need for laboratory handling procedures.
At present, there is a growing demand for developing minimal–or non–invasive implantable devices able to measure in real–time with high Author: P. Salazar.Glutamate, for example, must be detected in the brain at levels of 1–10 lm in the presence of – lm ascorbate. Ascorbate oxidase can be used to eliminate the ascorbate interference .
Unlike most oxidases, peroxide is not a product of the reaction and therefore does not interfere with glutamate detection Cited by: Real-time monitoring of neurotransmitters is of utmost importance for understanding the functioning of the brain. Especially L-glutamate and choline play a major role in chemical signalling and are highly involved in cognitive functions such as learning, memory and behaviour.
In this thesis, silicon microprobe arrays were realised comprising several microelectrodes with a size of 50× : Olivier Frey.